目的:提取并纯化粉防己多糖(SMP),确定其组成,研究其清除活性氧自由基的作用。方法:利用水提醇沉得到粉防己粗多糖,再经DEAE纤维素和聚酰胺纯制。利用Fenton反应研究SMP清除.OH的作用;利用邻苯三酚自氧化法研究SMP清除.O2-的作用。结果:纸色谱显示SMP是由葡萄糖组成的匀多糖;SMP清除.OH的能力较弱;清除.O2-时的效果稍强,IC50为1956.1μg/ml。结论:粉防己多糖为首次提取制得,有一定的清除羟自由基和超氧阴离子自由基的作用。 OBJECTIVE: To extract and purify Tetranychus polysaccharide (SMP), determine its composition, and study its role in scavenging reactive oxygen species. Methods: The water extracted alcohol precipitation was used to obtain the powder-adjusted crude polysaccharide, and then purified by DEAE cellulose and polyamide. The Fenton reaction was used to study the effect of SMP on the removal of .OH; the pyrolysis of pyrogallol was used to investigate the role of SMP in removing .O2-. RESULTS: Paper chromatography showed that SMP was a homogenous polysaccharide composed of glucose; SMP had a weaker ability to remove .OH; the effect of removing O2- was slightly stronger, and the IC50 was 1956.1 μg/ml. Conclusion: Tetrandopsa polysaccharides were prepared for the first time and had certain effects of scavenging hydroxyl radicals and superoxide anion radicals.