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[目的]为研究朽木生态小环境中各种微生物之间的相互作用和农业废弃秸秆的再利用奠定基础。[方法]利用分离的几株来源于云贵高原地区次生林朽木中具有较高纤维素分解活性的细菌(B2、Q1)、真菌(W9)与放线菌(H1)分别和混合发酵纤维素羧甲基纤维素钠和滤纸等底物,研究碳源种类、培养时间对纤维素分解酶活的影响。[结果]分离的4株菌及混合菌在4种碳源上均能良好生长,其中B2和H1在羧甲基纤维素钠为碳源培养72h的纤维素酶活性最高,分别为61.9 U和57.7U;混合菌在各种碳源中发酵活力均较低;混合发酵较不同菌株单独发酵产生还原糖总量略有降低;在以玉米秸秆为唯一碳源的培养基上各种菌的纤维素分解酶活性随培养时间的延长逐渐增加。[结论]混合菌的纤维素分解活性均低于各种菌单独发酵。
[Objective] The research aimed to establish the foundation for studying the interaction between various microorganisms in the ecological environment of turfgrass and the reuse of agricultural waste straw. [Method] Several isolated strains (B2, Q1), fungi (W9) and actinomycetes (H1) derived from secondary forest rotten wood in the Yunnan - Guizhou Plateau were mixed with carboxymethylcellulose Cellulose sodium and filter paper and other substrates to study the carbon source types, culture time on the cellulolytic enzyme activity. [Result] The four isolates and mixed bacteria could grow well on all four kinds of carbon sources. Among them, B2 and H1 had the highest activity of cellulase at 72h with sodium carboxymethyl cellulose as carbon source, which were 61.9 U and 61.9 U, respectively 57.7U. The viability of the mixed bacteria was low in all kinds of carbon sources. The total amount of reducing sugars produced by the mixed fermentation was slightly lower than that of the different strains. In the medium with corn stalk as sole carbon source, The activity of proteolytic enzyme increased gradually with the prolongation of culture time. [Conclusion] The cellulolytic activity of the mixed bacteria was lower than that of various strains alone.