测定胃蛋白酶是我组研究脾气虚证的一项指标,而目前国内对人和动物胃液中胃蛋白酶的测定方法尚很少报导。我组在造成家兔脾气虚时,对家兔胃液中胃蛋白酶进行了测定。其方法是在人的尿蛋白酶测定方法的基础上改进的,现介绍如下。一、测定原理以血红蛋白为基质,在胃蛋白酶的催化下释放出酪氨酸。在再加 CCl_3COOH 沉淀蛋白质,上清液即含酪氨酸。在硷性环境中, The determination of pepsin is an indicator of the spleen-qi deficiency syndrome in our group. However, there are few reports on the determination of pepsin in gastric juice in humans and animals. When my group caused rabbit spleen qi deficiency, it measured pepsin in the rabbit’s gastric juice. The method is based on the improvement of the human urinary protease assay method and is described below. First, the principle of measurement to hemoglobin as a substrate, under the catalysis of pepsin release tyrosine. Proteins were precipitated by the addition of CCl 3 COOH, and the supernatant contained tyrosine. In an embarrassing environment,