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本试验以‘津春4号’黄瓜幼苗叶片为试材,研究HCO3-、K+和HSO3-对黄瓜幼苗光合作用的影响,试图用KHCO3水溶液中的HCO3-作为碳源来补充CO2的不足,同时用NaHSO3适当的抑制黄瓜的光呼吸,进而提高光合速率。结果表明:HCO3-可以作为碳源来补充大气中CO2的不足,HCO3-、K+和HSO3-可以提高光合速率、叶片可溶性糖含量,可提高叶绿素a含量、叶绿素b含量及叶绿素总含量,从而增强了光合作用的原初反应,能显著提高PSI和PSII的光合电子传递速率,提高ATP合酶的活性,从而加快了光合磷酸化的进程。通过提高Rubisco羧化活性、PEPC酶的含量及活性,降低Rubisco加氧活性,加快了CO2的固定与还原。
In this experiment, the effect of HCO3-, K + and HSO3- on the photosynthesis of cucumber seedlings was studied with ’Jinchun 4’ cucumber seedling leaves as an experimental material, trying to make up for the lack of CO2 by using HCO3- in the aqueous KHCO3 solution as a carbon source, With NaHSO3 appropriate inhibition of photorespiration of cucumbers, thereby increasing photosynthetic rate. HCO3-, K + and HSO3- could increase photosynthetic rate, leaf soluble sugar content, increase chlorophyll a content, chlorophyll b content and total chlorophyll content, and thus enhance The initial photosynthesis reaction can significantly increase the photosynthetic electron transfer rate of PSI and PSII and increase the activity of ATP synthase, thus accelerating the process of photosynthetic phosphorylation. By increasing the carboxylation activity of Rubisco, the content and activity of PEPC enzyme, reducing the activity of Rubisco and accelerating the fixation and reduction of CO2.