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应用抗细胞角蛋白单克隆抗体ＡＥ１／ＡＥ３，对食管癌、胃癌和大肠癌的原发组织反应进行了研究。结果表明，４６例癌组织的反应率是９３％，食管癌为１００％，胃癌和大肠癌分别是９４％和７９％；反应强度和反应范围显示，食管癌较胃和大肠癌好。ＡＥ１／ＡＥ３符合做检测食管癌在非上皮组织（淋巴结、骨髓和外周血）中的微小转移的探针，不宜用来检测胃和大肠癌的微小转移，至少不能单独使用。同为胃和大肠来源的腺癌组织可出现阴性反应，说明组织来源完全相同的肿瘤可有不同的ＣＫ亚型。对抗某一个或几个ＣＫ亚型单克隆抗体阴性反应的肿瘤，不能完全除外为上皮来源。鉴别疑难肿瘤的组织来源时，必须应用多个抗ＣＫ的单克隆抗体，并且结合其他标记才更有临床意义。
The primary tissue response of esophageal cancer, gastric cancer and colorectal cancer was studied using anti-cytokeratin monoclonal antibody AE1/AE3. The results showed that the response rate was 93% in 46 cases of cancer, 100% in esophageal cancer, 94% in gastric cancer and 79% in colorectal cancer, and the response intensity and response range showed that esophageal cancer was better than stomach and colon cancer. AE1/AE3 is suitable for detecting micrometastasis in esophageal cancer in non-epithelial tissues (lymph nodes, bone marrow, and peripheral blood) and should not be used to detect micrometastases in gastric and colorectal cancer, at least not alone. A negative reaction may occur in both adenocarcinoma tissues of the stomach and large intestine, indicating that tumors of the same origin may have different CK subtypes. Tumors that are negatively reactive against one or more CK subtype monoclonal antibodies cannot be completely excluded from epithelial origin. When identifying tissue sources of difficult tumors, multiple monoclonal antibodies against CK must be used, and combining other markers is more clinically meaningful.