论文部分内容阅读
目的:建立鼻炎康颗粒的质量标准研究方法。方法:采用TLC法鉴别制剂中的黄芩和葛根;采用HPLC法测定黄芩苷的含量,色谱柱为Dubhe C18柱(250 mm×4.6 mm,5“m),以甲醇为流动相A,0.4%磷酸溶液为流动相B进行梯度洗脱,流速为1.0 ml·min~(-1),检测波长为280 nm,柱温为30℃,进样量为10”l。结果:各供试品的TLC图谱显示,在与对照品或对照药材相应的位置上显相同颜色的斑点,阴性对照无干扰;黄芩苷在15.02~300.43"g·ml-1浓度范围内与峰面积呈良好的线性关系(r=1.000 0),平均回收率为102.62%(RSD=0.72%,n=6)。结论:在鼻炎康颗粒原标准基础上建立的方法具有专属性强、准确度高、重复性好的特点,可有效控制该制剂的质量。
Objective: To establish a quality standard research method of rhubarb granules. Methods: Scutellaria baicalensis and Radix Puerariae were identified by TLC method. The content of baicalin was determined by HPLC. The column was Dubhe C18 column (250 mm × 4.6 mm, 5 μm) with methanol as mobile phase A, 0.4% The mobile phase B was eluted with gradient elution of phosphoric acid solution at a flow rate of 1.0 ml · min -1 with a detection wavelength of 280 nm and a column temperature of 30 ° C. Results: The TLC images of the test samples showed that the same color spots were found on the corresponding sites of the reference substance or the reference drug, and the negative control had no interference. The concentration of baicalin in the range of 15.02-300.43 g · ml-1 (R = 1.000 0), the average recovery was 102.62% (RSD = 0.72%, n = 6) .Conclusion: The method established on the basis of rhubarb granule standard has the characteristics of strong specificity and accuracy High degree of repeatability and good features, which can effectively control the quality of the preparation.