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目的探讨诱导大鼠原位膀胱癌动物模型早期应用雌激素干预,观察其膀胱的成瘤情况、肿瘤大小及病理分级有无影响。方法给切除卵巢的SD大鼠行N-甲基亚硝基脲(MNU)膀胱灌注,每次2mg/只,1次/2周,共4次,以制备大鼠原位膀胱癌动物模型。随机分成雌激素提前干预、雌激素非提前干预和仅灌注MNU三组相比较。结果应用雌激素干预的二组与仅灌注MNU组相比较,在成瘤率、肿瘤大小及方面差异均有统计学意义(P<0.01);雌激素提前干预和雌激素非提前干预二组间比较差异无统计学意义(P>0.05),但雌激素提前干预组肿瘤发生率更低。结论化学致癌剂N-甲基亚硝基脲(MNU)经膀胱灌注可成功建立原位膀胱癌动物模型,经雌激素提前干预明显降低膀胱癌的成瘤率及抑制肿瘤生长,为膀胱癌内分泌治疗提供佐证。
Objective To investigate the early application of estrogen in rat model of orthotopic bladder cancer and to observe its tumorigenicity, tumor size and pathological grade. Methods The ovariectomized SD rats were subjected to intravesical instillation of 2-methyl-N-nitrosourea (MNU) intraperitoneally (2mg / time, once / 2 weeks) for 4 times to prepare the rat model of orthotopic bladder cancer. Randomly divided into estrogen intervention in advance, estrogen non-intervention and only MNU perfusion compared to the three groups. Results Compared with MNU group, there was significant difference in tumor formation rate, tumor size and aspect between the two groups treated with estrogen (P <0.01); the early estrogen intervention and estrogen non-intervention The difference was not statistically significant (P> 0.05), but the estrogen pretreatment group had a lower incidence of cancer. Conclusion The chemical carcinogen N-methylnitrosourea (MNU) can successfully establish an in situ bladder cancer animal model by intravesical instillation of bladder cancer. Early intervention with estrogen can significantly reduce the tumorigenic rate and tumor growth of bladder cancer, Treatment provides evidence.