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近年来由于细胞生物学,免疫学和细胞化学的发展,使病理学的研究超越了传统的实验范畴,在理论上方法上有很大的发展,然而常规的病理学切片技术仍是临床和科研工作中常用的主要方法。由于科研工作的需要,不同种类实验动物的组织石蜡切片应用日益广泛。实验动物组织柔软,含水份多,在制片过程中固定,脱水,透明和浸蜡容易出现组织块变脆,影响切片的成功率。我室通过多年的工作实践,对以上问题进行了研究和改进,报告如下。
In recent years, due to the development of cell biology, immunology and cytochemistry, the research of pathology has gone beyond the traditional experimental field and there has been a great development in the theoretical methods. However, the conventional pathological sectioning technique is still clinical and scientific The main method commonly used in work. Due to the need of scientific research, tissue paraffin sections of different kinds of experimental animals are widely used. Experimental animal tissue soft, moisture content and more fixed during the production process, dehydration, transparency and waxing prone to brittle tissue block, affecting the success rate of slicing. My room through many years of work practice, the above problems have been studied and improved, the report is as follows.