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目的检测癌前病变和不同分化阶段胃癌中E-cadherin基因甲基化状态、mRNA和其蛋白表达情况。方法应用甲基化特异性PCR(methylation-specific PCR,MSP)分析、原位杂交和免疫组化等方法进行研究。结果癌前病变组织中E-cadherin DNA甲基化阳性表达率高于正常胃黏膜组织,差异有统计学意义(P<0.05);E-cadherin mRNA在正常胃黏膜中的表达高于癌前病变组织,差异有统计学意义(P<0.05);E-cadherin蛋白表达在正常胃黏膜中的表达高于癌前病变组织,差异有统计学意义(P<0.05)。E-cadherin DNA甲基化、E-cadherin mRNA及E-cadherin蛋白均与胃癌的分化程度相关。结论 E-cadherin基因高甲基化可能引起E-cadherin mRNA和蛋白表达减低,使其失去细胞粘附作用,从而增加了胃癌恶变的风险。
Objective To detect the methylation status, mRNA and protein expression of E-cadherin gene in precancerous lesions and gastric cancer at different stages of differentiation. Methods Methylation-specific PCR (MSP) analysis, in situ hybridization and immunohistochemistry were used to study the effects of these drugs. Results The positive rate of E-cadherin DNA methylation in precancerous lesions was significantly higher than that in normal gastric mucosa (P <0.05). The expression of E-cadherin mRNA in normal gastric mucosa was higher than that in precancerous lesions The difference was statistically significant (P <0.05). The expression of E-cadherin protein in normal gastric mucosa was higher than that in precancerous lesions, the difference was statistically significant (P <0.05). E-cadherin DNA methylation, E-cadherin mRNA and E-cadherin protein were related to the degree of differentiation of gastric cancer. Conclusion The hypermethylation of E-cadherin gene may result in the decrease of E-cadherin mRNA and protein expression and the loss of cell adhesion, thus increasing the risk of malignant transformation of gastric cancer.