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目的建立胞液型磷脂酶A2（cytosolicphospholipaseA2,cPLA2）的ELISA。方法以人cPLA2抗体包被微孔板后，加入抗原（标本），再加入辣根过氧化物酶标记的羊抗人IgG作为第二抗体，经邻苯二胺（OPD）显色后测定其490nm处A值，与标准品对比可得cPLA2含量。结果检测灵敏度可达25．0ng／ml，批内和批间变异系数为5．5％和7．8％，cPLA2含量为56和120mg／ml标本的平均回收率均大于80％。结论该方法是一种测定cPLA2含量的简便可靠方法。
Objective To establish an ELISA for cytosolic phospholipase A2 (cPLA2). Methods Human cPLA2 antibody was used to coat microtiter plate wells, antigens (samples) were added, and horseradish peroxidase labeled goat anti-human IgG was added as the secondary antibody. After OPD staining, 490nm at A value, compared with the standard cPLA2 content available. Results The detection sensitivity was up to 25.0 ng / ml, the intra-assay and inter-assay CVs were 5.5% and 7.8%, and the average recoveries of cPLA2 at 56 and 120 mg / ml were greater than 80%. Conclusion This method is a simple and reliable method for the determination of cPLA2 content.