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目的 探索建立颅咽管瘤体外模型的方法 ,寻找博莱霉素治疗颅咽管瘤的理论依据。方法 取 5人份新鲜颅咽管瘤组织块 ,以胶原酶消化成单细胞悬液 ,进行体外培养 ,在不同时段计数以画出生长曲线 ;加入 0 0 1mg/ml,0 1mg/ml和 1mg/ml等含博莱霉素的不同浓度培养液培养 ,在不同时段计数以画出抑瘤曲线 ,并取加药培养 6天后细胞以ATP荧光测定法测定抑瘤效果。结果 4人份组织块短期培养成功 ,细胞数随时间延长而下降 ,加药后下降更明显 ,且浓度越高下降越明显 ,ATP荧光测定法显示 ,0 1mg/ml和 1mg/ml浓度组有显著抑瘤效应 ,而 0 0 1mg/ml组则耐药。结论 以消化法可成功进行颅咽瘤短期体外培养 ,并用于建立体外模型 ,博莱霉素可抑制颅咽管瘤体外细胞 ,存在浓度和时间依赖效应 ,ATP荧光测定法用于药敏实验敏感、准确 ,有推广价值
Objective To explore the method of establishing an in vitro model of craniopharyngioma and to find the theoretical basis for the treatment of craniopharyngioma with bleomycin. Methods Five fresh craniopharyngioma tissue masses were obtained and digested with collagenase into single cell suspension for in vitro culture. The growth curves were counted at different time points. Growth curves were drawn with 0 0 1 mg / ml, 0 1 mg / ml and 1 mg / ml and other bleomycin containing different concentrations of culture medium, counting at different times to draw tumor inhibition curve, and take drug dosing 6 days after the cells to determine the inhibitory effect of ATP fluorescence assay. Results The tissue culture of 4 human tissues was successfully cultured in short term and the number of cells decreased with time. The decrease of the number of cells was more obvious with the increase of time. The higher the concentration, the more obvious the decrease was. ATP fluorescence assay showed that the concentration of 0 1mg / ml and 1mg / ml Significant tumor inhibitory effect, while 0 0 1mg / ml group is resistant. CONCLUSIONS: Short-term in vitro culture of craniopopharyngoma can be successfully established by digestion and used to establish an in vitro model. Bleomycin inhibits craniopharyngioma cells in vitro in a concentration- and time-dependent manner. ATP-fluorometric assay is sensitive to susceptibility testing , Accurate, there are promotional value