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目的:分析来自黄疸和先天性巨结肠(hirschsprung’s disease,HD)患儿的人巨细胞病毒临床分离株UL141片段的序列变化,以及这种变化与人巨细胞病毒感染导致不同消化系疾病之间的关系.方法:对荧光定量PCR方法检测HCMV-DNA阳性的临床低传代分离株进行UL141基因全序列PCR扩增,对扩增阳性的标本进行测序及分析.结果:15株临床低传代分离株在Toledo株UL141基因227位均缺失一个碱基T,因此产生两个新的ORF(UL141A和UL141B).与Toledo株相应片段比较,UL141A预测蛋白质第75位氨基酸后序列和翻译后修饰位点产生大量变异.UL141B的核苷酸和氨基酸序列均高度保守.结论:来自黄疸和先天性巨结肠患儿的人巨细胞病毒临床分离株的UL141片段产生两个新的ORF,这两个ORF在黄疸和先天性巨结肠分离株之间均未发现特异性变异
OBJECTIVE: To analyze the sequence variations of the human cytomegalovirus clinical isolate UL141 from jaundice and children with congenital hirschsprung’s disease (HD) and the relationship between this change and the human cytomegalovirus infection leading to different digestive diseases Relationship.Methods: PCR-based PCR was used to detect the HCMV-DNA positive low-passage isolates, and the UL141 gene was sequenced and analyzed by PCR.Results: Among the 15 clinical low-passage isolates, Two new ORFs (UL141A and UL141B) were generated in the Toledo strain, the 227 locus of UL141 gene.Compared with the corresponding fragment of Toledo strain, UL141A predicted that the 75th amino acid sequence and posttranslational modification sites of the protein produced a large amount of Mutation.UL141B nucleotide and amino acid sequences were highly conserved.Conclusion: UL141 fragment of human cytomegalovirus clinical isolates from jaundice and Hirschsprung’s disease patients produce two new ORF, the two ORF in jaundice and No specific mutation was found between Hirschsprung strains