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目的:探讨食管鳞状细胞癌(ESCC)组织中Ezrin蛋白与E-cadherin的表达及其与肿瘤侵袭和转移的关系。方法:以30例癌旁正常黏膜组织作对照,应用免疫组织化学SP法检测76例ESCC组织标本中Ezrin蛋白与E-cadherin的表达。结果:ESCC组织中Ezrin蛋白与E-cadherin的异常表达率分别为78.95%和59.21%;而癌旁正常黏膜组织中异常表达率分别为26.67%和6.67%,两项指标差异均有统计学意义(P<0.01)。Ezrin蛋白异常表达在ESCC淋巴结转移和组织分化程度间差异有统计学意义(P<0.05和P<0.01),E-cadherin异常表达在ESCC组织的浸润深度、ESCC细胞的分化程度和淋巴结转移间差异均有统计学意义(P<0.05~P<0.01)。Ezrin蛋白与E-cadherin在ESCC中的异常表达呈正相关关系(P<0.05)。Ecadherin异常表达组Ezrin蛋白表达阳性率显著高于E-cadherin正常表达患者(P<0.05)。两者同时异常表达的40例中淋巴结转移高达70%,而两者同时正常表达的11例均无淋巴结转移,差异有统计学意义(P<0.01)。结论:Ezrin蛋白与Ecadherin在ESCC组织中均异常表达,且呈正相关。联合检测Ezrin蛋白和E-cadherin的表达有助于综合判断ESCC的浸润、转移及预后。
Objective: To investigate the expression of Ezrin protein and E-cadherin in esophageal squamous cell carcinoma (ESCC) and its relationship with tumor invasion and metastasis. Methods: Thirty normal paracancerous mucosa tissues were used as control. The expression of Ezrin protein and E-cadherin in 76 ESCC tissues were detected by immunohistochemical SP method. Results: The abnormal expression rates of Ezrin protein and E-cadherin in ESCC tissues were 78.95% and 59.21%, respectively. The abnormal expression rates in normal mucosa tissue were 26.67% and 6.67%, respectively (P <0.01). Ezrin protein expression in ESCC lymph node metastasis and differentiation between the degree of differentiation was statistically significant (P <0.05 and P <0.01), E-cadherin abnormal expression in ESCC tissue depth of invasion, ESCC cell differentiation and lymph node metastasis All were statistically significant (P <0.05 ~ P <0.01). The abnormal expression of Ezrin protein and E-cadherin in ESCC was positively correlated (P <0.05). The positive rate of Ezrin protein expression in Ecadherin abnormal expression group was significantly higher than that in E-cadherin normal expression group (P <0.05). There was no lymph node metastasis in all of the 40 cases with abnormal expression of both of them, but there were no lymph node metastasis in the 11 cases with both of them (P <0.01). Conclusion: Ezrin protein and Ecadherin are both abnormally expressed in ESCC tissues and positively correlate with them. Joint detection of Ezrin protein and E-cadherin expression contribute to a comprehensive assessment of ESCC infiltration, metastasis and prognosis.