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目的:探讨胃癌组织中Survivin、Cox-2与HIF-1α蛋白的表达及其临床意义。方法:采用免疫组织化学染色法检测Survivin、Cox-2及HIF-1α在65例胃癌组织、18例癌旁组织及20例正常胃黏膜的表达水平,并分析其与胃癌生物学行为的关系。结果:Survivin、Cox-2与HIF-1α在正常胃黏膜、癌旁组织、癌组织中的表达呈升高趋势,其差异均有统计学意义(P<0.05)。Survivin阳性表达在TNM分期、组织学分化程度、胃癌浸润深度、淋巴结转移及远隔转移方面差异显著(P<0.05);Cox-2阳性表达在TNM分期、组织学分化程度和淋巴结转移方面差异显著(P<0.05);HIF-1α阳性表达在TNM分期、组织学分化程度、胃癌浸润深度和淋巴结转移方面差异显著(P<0.05)。相关分析显示,65例胃癌组织中,Survivin、Cox-2与HIF-1α表达均呈正相关(r=0.285,0.405,0.546,P<0.05)。结论:Survivin、Cox-2与HIF-1α的过表达在胃癌的发生、发展、浸润和转移过程中发挥协同作用,可成为胃癌基因诊断和治疗的新靶点。
Objective: To investigate the expression of Survivin, Cox-2 and HIF-1α in gastric cancer and its clinical significance. Methods: The expressions of Survivin, Cox-2 and HIF-1α in 65 gastric cancer tissues, 18 adjacent tissues and 20 normal gastric mucosa were detected by immunohistochemical staining. Results: The expressions of Survivin, Cox-2 and HIF-1alpha in normal gastric mucosa, paracancerous tissues and cancerous tissues showed an increasing tendency, with statistical significance (P <0.05). The positive expression of Survivin in TNM staging, histological differentiation, gastric cancer invasion depth, lymph node metastasis and distant metastasis were significantly different (P <0.05); Cox-2 positive expression in TNM staging, histological differentiation and lymph node metastasis were significantly different (P <0.05). The positive expression of HIF-1α in TNM staging, histological differentiation, gastric cancer invasion depth and lymph node metastasis were significantly different (P <0.05). Correlation analysis showed that there was a positive correlation between Survivin, Cox-2 and HIF-1α expression in 65 cases of gastric cancer (r = 0.285,0.405,0.546, P <0.05). CONCLUSIONS: Survivin, Cox-2 and HIF-1α overexpression play a synergistic role in the genesis, development, invasion and metastasis of gastric cancer, which may be a new target for gene diagnosis and treatment of gastric cancer.