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研究了Pr(Ⅲ ) 杯 [4 ]芳烃 DNA体系的相互作用和荧光发光情况。试验结果表明 ,Pr(Ⅲ )可产生λex ,max=30 0 .0nm、λem ,max=60 0 .0nm的自发荧光 ,杯 [4]芳烃在一定条件下能猝灭其荧光 ,加入DNA后荧光产生大幅增强 ,故可以杯 [4]芳烃与Pr(Ⅲ )形成的发光镧系超分子作荧光探针测定DNA ,同时初步探讨了体系相互作用机理。试验方法的线性范围为 0～ 1.0 μg·ml- 1和 2 .0～ 4 .0 μg·ml- 1,检出限为 1.2 76× 10 - 3μg·ml- 1。此法简单、灵敏、可靠
The interaction and fluorescence of Pr (Ⅲ) calix [4] arene DNA system were studied. The experimental results show that Pr (Ⅲ) can generate autofluorescence with λex, max = 30 0 .0nm, λem, max = 60 0 .0nm. Calix [4] arene can quench its fluorescence under certain conditions. Therefore, it is possible to determine DNA by luminescent lanthanide supramolecular complexes formed by calix [4] arene and Pr (Ⅲ). At the same time, the interaction mechanism of the system was preliminarily discussed. The linear range of the experimental method was 0-1.0 μg · ml-1 and 2.0-0.4 μg · ml-1 with a detection limit of 1.276 × 10-3 μg · ml-1. This method is simple, sensitive and reliable