[Objective] To establish the technical parameters,enrichment and purification procedures of total glycosides from Acanthopanax giraldii Harms with macroporous resin. [Methods]A RP-HPLC method was adopted to determine the content of total glycosides in A. giraldii Harms,and with the elution efficiency of syringin and oleanolic acid as the index,the adsorption characteristics and elution parameters of the enrichment and purification procedures were tested from 10 kinds of macroporous resin. [Results] The method was good for determining the content of total glycosides. X-5 was the best of macroporous resin; the optimal concentration of total glycosides solution extracted from A. giraldii Harms was 0. 30 g crude powder / m L; the best p H of the powder solution was 10; static adsorption lasted for 8 h; the best dosage of 80%ethanol was 4 BV. [Conclusions]These results were accurate and repeatable; X-5 macroporous resin was the best one for enrichment and purification procedures of total glycosides from A. giraldii Harms.