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目的为了解济宁地区献血人群中人细小病毒B19(human parvovirus B19,简称HPV B19)感染情况,探讨血浆合并对其检出率的影响情况,降低临床输血风险提供一定依据。方法采用酶联免疫吸附法(ELISA)和实时荧光定量PCR(RTPCR)法对采集的960份献血员血液样本进行单人份及随机8人份血浆合并样本进行HPV B19-Ig G、Ig M抗体及DNA检测。结果单人份血浆与随机8人份血浆合并样本HPV B19 Ig G抗体、Ig M抗体阳性率分别为36.15%、5.94%、35.83%、12.50%,抗体阳性标本中单人份及血浆合并样本DNA阳性率分别为0.83%、5.00%。其中Ig M抗体阳性组B19 DNA检出率明显高于Ig G阳性组。结论献血人群中存在较高的HPV B19感染率,血浆合并后Ig M抗体及DNA检出率较高,未提示其传播风险降低,应对儿童、老年人、孕妇等免疫功能低下者选择性输注B19-Ig G抗体滴度较高的血液制品。
Objective To investigate the prevalence of human parvovirus B19 (B19) infection in blood donors in Jining, and to explore the impact of plasma pooling on the detection rate and to provide a basis for reducing the risk of clinical transfusion. Methods A total of 960 blood samples from blood donors collected from single samples and random samples of 8 human plasma samples were detected by enzyme-linked immunosorbent assay (ELISA) and real-time fluorescence quantitative PCR (RTPCR) for HPV B19-Ig G and Ig M antibodies And DNA testing. Results The positive rates of HPV B19 Ig G antibody and Ig M antibody in single sample plasma and random sample of 8 human plasma samples were 36.15%, 5.94%, 35.83% and 12.50%, respectively. The positive rates of single antibody and plasma sample DNA The positive rates were 0.83% and 5.00% respectively. The positive rate of B19 DNA in IgM positive group was significantly higher than that in IgG positive group. Conclusion There is a high prevalence of HPV B19 infection in blood donors. The higher detection rate of IgM antibody and DNA after plasma combination does not suggest that it has a lower risk of transmission, and should be used for selective infusion of immunocompromised children, such as children, the elderly and pregnant women B19-Ig G antibody titers higher blood products.