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研究了温度、pH值、光照、碳源、氮源和培养基种类对菜心 (BrassicaparachinensisBailey)炭疽病菌(ColletotrichumhigginsianumSacc .)菌丝生长、产孢和孢子萌发的影响。结果表明 :菌丝生长和产孢的温度均为 8～ 35℃ ,最适温度均为 2 5℃ ;在 10～ 35℃范围内 ,病菌的分生孢子均可萌发 ,最适温度为 2 5℃ ;分生孢子的致死温度为 5 3℃ ,10min或 5 5℃ ,5min。病菌在pH 3 0～ 10 0均能生长及产孢 ,最适pH值均为 6 0。光照对菌丝的生长速率影响不大 ,但连续光照有利于菌丝干物质的积累和孢子量的增加。在被测试的 9种碳源中 ,菌丝生长速率以甘露醇、麦芽糖和葡萄糖为最快 ;菌丝干重以葡萄糖为最高 ;产孢量以麦芽糖为最多。在被测试的 9种氮源中 ,菌丝生长以甘氨酸为最好 ;菌丝干重以硝酸钾为最好 ,产孢量以尿素为最好。在被测试的 11种培养基中 ,菌丝生长以PDA和菜心榨汁PDA培养基为最好 ;产孢量以玉米粉琼脂培养基为最多
The effects of temperature, pH value, light, carbon source, nitrogen source and medium on mycelial growth, sporulation and spore germination of Colletotrichum baigiiianger.Sacc. Were studied. The results showed that the temperature of mycelial growth and sporulation were both 8 ~ 35 ℃ and the optimum temperature was 25 ℃. Conidiospores germinated in the range of 10 ~ 35 ℃ with the optimum temperature of 25 ℃; the lethal temperature of conidia is 5 3 ℃, 10min or 5 5 ℃, 5min. The pathogen could grow and produce spores at the pH range of 30 ~ 100, and the optimum pH value was 60. Light had little effect on the growth rate of mycelium, but continuous light was beneficial to the accumulation of dry matter and the increase of spore amount. Among the nine carbon sources tested, the mycelial growth rate was the fastest with mannitol, maltose and glucose; the dry weight of mycelia was the highest with glucose; the spore yield was maltose. Among the nine nitrogen sources tested, mycelial growth was best with glycine; dry potassium nitrate was the best dry mycelium and urea was the best sporulation. Among the eleven kinds of media tested, mycelial growth was the best in PDA culture medium of PDA and Brassica juncea; sporulation volume was the most in cornmeal agar