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采用光敏核不育水稻(HPGMR)成熟胚为外植体,在不同植物激素浓度和配比下诱导愈伤组织,并观察再生植株的发生特点和过程,同时分析愈伤组织酯酶和过氧化物酶同工酶谱的变化特征,初步表明诱导和分化频率较高的植物生长素与细胞激动素的配比.
The mature embryos of photoperiod-sensitive genic male-sterile rice (HPGMR) were used as explants. The callus was induced under different plant hormones concentration and ratio, and the occurrence and process of regenerated plants were observed. Meanwhile, the esterase and peroxidase The changes of the isoenzyme zymogram of the isozyme initially indicated that the auxin and cytokinin ratio of the higher induction and differentiation frequency.