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目的建立RP-HPLC同时测定不同产地叶下珠中没食子酸、柯里拉京、老鹳草素和短叶苏木酚酸的方法。方法采用Diamonsil C18柱(250 mm×4.6 mm,5μm),以乙腈-0.2%醋酸水溶液为流动相,梯度洗脱,检测波长为270 nm,体积流量1 mL/min,柱温35℃。结果没食子酸、柯里拉京、老鹳草素和短叶苏木酚酸的线性范围分别为0.062 2～0.622 0μg(r=0.999 7)、0.130 4～1.304 0μg(r=0.999 4)、0.246 4～2.464 0μg(r=0.999 2)、0.081 2～0.488 0μg(r=0.999 3);平均回收率分别为103.62%、101.20%、100.09%、100.27%,RSD分别为1.29%、2.08%、1.40%、2.98%。结论本测定方法简便、准确、重复性好,可用于叶下珠中没食子酸、柯里拉京、老鹳草素和短叶苏木酚酸的测定。
OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of gallic acid, corilagin, prunella and short-leaf thymol in Phyllanthus urinaria from different habitats. Methods Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile - 0.2% acetic acid aqueous solution. The detection wavelength was 270 nm, the volume flow rate was 1 mL / min and the column temperature was 35 ℃. Results The linear ranges of gallic acid, corilagin, prunella and short-leaf thymol were 0.062 2 ~ 0.622 0μg (r = 0.999 7), 0.1304 ~ 1.304 0μg (r = 0.999 4), 0.2464 ~ 2.464 The average recoveries were 103.62%, 101.20%, 100.09% and 100.27%, respectively, with RSDs of 1.29%, 2.08%, 1.40% and 2.98 %. Conclusion The method is simple, accurate and reproducible. It can be used for the determination of gallic acid, corilagin, prunella and short-leaf hesperidic acid in Phyllanthus.