论文部分内容阅读
目的:探讨在B超引导下经皮穿刺建立大鼠种植性肝癌模型的可行性及稳定性。材料和方法:52只SD大鼠在B超引导下将浓缩的Walker-256瘤细胞混悬液注入大鼠肝左叶,观察种植的成功率、肿瘤的生长方式、生长速度、B超表现。结果:52只大鼠造模成功51只,成功率98.1%,肿瘤生长方式多为肝内圆形或类圆形单发结节状,B超表现可为高、等高或低回声。个别肿瘤长出肝外或引起癌性腹水。造模3、6、9、12d后肿瘤最大径依次为0.2、0.6、0.9、1.4cm,肿瘤体积依次为0.004±0.001、0.075±0.012、0.288±0.037、1.183±0.215cm3。结论:B超引导下建立大鼠Walker-256种植性肝癌模型具有简单、微创、成功率高等特点,值得推广利用。
Objective: To investigate the feasibility and stability of percutaneous needle puncture under the guidance of B-ultrasound in rat model of hepatocellular carcinoma in rats. MATERIALS AND METHODS: Thirty-two Sprague-Dawley rats were injected with the concentrated Walker-256 tumor cell suspension into the left lobe of liver under B-ultrasound guidance. The success rate of implantation, tumor growth, growth rate and B ultrasound were observed. Results: Fifty-two rats were successful in modeling 51 with a success rate of 98.1%. Most of the tumor growth patterns were intrahepatic or quasi-circular single nodules. B-ultrasound showed high, high or low echogenicity. Some tumors grow extrahepatic or cause cancerous ascites. After 3, 6, 9 and 12 days, the maximal diameters of tumors were 0.2,0.6,0.9 and 1.4cm, and the tumor volumes were 0.004 ± 0.001,0.075 ± 0.012,0.288 ± 0.037,1.183 ± 0.215cm3. Conclusion: The Walker-256 implanted hepatocellular carcinoma model established by B-ultrasound is simple, minimally invasive and has a high success rate. It is worth popularizing and utilizing.