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目的 研究尿激酶型纤溶酶原激活因子 (u PA)与人脑胶质瘤恶性生物学行为的关系 .方法 采用 Northern印迹分子杂交和免疫组化方法检测 43例人脑胶质瘤和 5例正常脑组织 u PA m RNA及蛋白的表达 ,并与临床生物学参数进行综合分析 .结果 上述组织或细胞均表达 2 .5 kb的 u PA m R-NA转录物 ,高级别胶质瘤较低级别和正常脑组织 u PA m R-NA表达水平明显增高 (P<0 .0 1 ) ,但低级别胶质瘤和正常脑组织 u PA m RNA表达水平无明显差异 (P>0 .0 5 ) .术后 1 8mo内复发和生存期小于 3a者 u PA m RNA表达显著高于术后 1 8mo无复发及生存期大于 3a者 (P<0 .0 1 ) ;靠近坏死区域的瘤细胞亦有 u PA m RNA表达 ,u PA m RNA表达水平与胶质瘤微血管数量呈正相关 (r=0 .5 6 ,P<0 .0 1 ) ,而与患者性别、年龄及肿瘤大小则无显著相关 .u PA蛋白主要分布在胶质母细胞瘤、间变性星形细胞瘤组织的瘤细胞及内皮细胞 ,低级别胶质瘤较少 .结论 u PA基因表达可能与胶质瘤的恶性发展有关 ,对预测高级别胶质瘤的侵袭性及术后复发方面具有重要作用
Objective To study the relationship between uPA and malignant biological behavior of human glioma.Methods 43 human gliomas and 5 human gliomas were detected by Northern blot hybridization and immunohistochemistry The expression of u PA m RNA and protein in normal brain tissue was analyzed and analyzed with clinical biological parameters.Results The above tissues or cells all expressed 2.5 u PA m R-NA transcripts, while the high grade gliomas were lower The level of u PA m R-NA was significantly higher in normal brain tissue and normal brain tissues (P <0.01), but there was no significant difference in u PA m RNA expression between low grade gliomas and normal brain tissues (P> 0.05) ) .The recurrence and survival time within 18 months after operation were lower than that of u PA m RNA expression was significantly higher than the postoperative 18m recurrence and survival of more than 3a (P <0.01); near the tumor necrosis area There was a positive correlation between u PA m RNA expression and u PA m RNA expression and glioma microvessel number (r = 0.56, P <0.01), but not with gender, age and tumor size .u PA protein is mainly distributed in glioblastoma, anaplastic astrocytoma tissue tumor cells and endothelial cells , Low-grade gliomas less.Conclusion u PA gene expression may be related to the malignant glioma development, which is important for predicting the aggressiveness and recurrence of high-grade gliomas