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目的 新建 1株人体肝细胞肝癌细胞系 ,为肝癌研究提供新的、理想的实验模型。 方法 以手术切除肝癌标本作原代培养 ,建立细胞系。通过光镜、电镜、倍增时间、生长曲线、双层软琼脂培养、流式细胞仪、染色体分析、刀豆球蛋白凝集试验、裸鼠接种、AFP、HBV及端粒酶检测 ,对其生物学特性进行分析。 结果 第 2 9天完成原代培养。形态学、增殖动力学及浸润性生长结果表明该细胞系符合恶性细胞特征。染色体数目分布在 49～ 135 ,众数为 6 0～ 6 7,1q(i) 和t(6 ,11)等为其标记染色体 ;有四射体和核内复制出现。裸鼠接种成瘤 ,瘤细胞形态与原病人的病理切片相似。端粒酶检测强阳性。 结论 该细胞系符合建系标准 ,是 1株新建的人体肝细胞肝癌细胞系 ,命名为HCC 990 3。其生物学特性研究内容及检测手段较国内外已建肝恶性肿瘤细胞系更为全面
Objective To establish a new human hepatocellular carcinoma cell line and provide a new and ideal experimental model for the study of liver cancer. Methods Surgical resection of liver cancer specimens was used as primary culture to establish cell lines. Through the light microscope, electron microscope, doubling time, growth curve, double soft agar culture, flow cytometry, chromosome analysis, concanavalin agglutination test, nude mice inoculation, AFP, HBV and telomerase detection, its biology Characteristics are analyzed. Results The primary culture was completed on the 29th day. Morphology, proliferation kinetics, and invasive growth results indicate that the cell line is consistent with malignant cell characteristics. Chromosome numbers range from 49 to 135, and frequencies from 60 to 617, 1q(i), and t(6, 11) are the marker chromosomes; there are tetramaroids and intranuclear replications. Nude mice were inoculated into tumors. The tumor cell morphology was similar to that of the original patient. Telomerase detection is strongly positive. Conclusion This cell line complies with the established standards and is a new human hepatocellular carcinoma cell line named HCC 990 3 . The research content and detection methods of biological characteristics are more comprehensive than those of established malignant tumor cell lines at home and abroad.