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目的:研究中药白术(Atractylodes macrocephala)对正常晚孕子宫平滑肌细胞及经白细胞介素6(Interleukin-6,IL-6)作用后的子宫平滑肌细胞钙依赖钾通道电流(BKca)的影响,以探讨白术对早产时宫缩的抑制作用及电生理机制。方法:①急性分离人晚孕子宫平滑肌细胞,采用Axopatchl-D膜片钳放大器测全细胞模式下的单个平滑肌细胞大电导的钙离子依赖钾通道电流(BKca);②测定灌流液中含不同浓度的白术时,子宫平滑肌细胞BKca的变化;③测定灌流液中含IL-6(10ng/ml)时全细胞模式下BKca;继而测定灌流液中含有不同浓度的白术时,子宫平滑肌细胞BKca的变化。结果:10ng/mlIL-6可抑制BKca的幅值约(36.9±13.7)%(n=10,P<0.01),灌流液中含2mg/ml白术时增强BKca的幅值约(36.7±22.6)%(n=10,P<0.01),对于IL-6作用后的细胞,2mg.mL-1白术增强BKca的幅值约(45.2±13.7)%(n=10,P<0.01)。结论:白术可以兴奋晚孕子宫平滑肌细胞及IL-6作用后的子宫平滑肌细胞的BKca,且对IL-6作用后的子宫平滑肌细胞BKca的兴奋作用强于对正常子宫平滑肌细胞,证明它有助于维持妊娠时子宫平滑肌的膜电位和静息状态,为开发中药治疗早产提供一定的理论依据。
AIM: To investigate the effect of Atractylodes macrocephala on calcium-dependent potassium channel current (BKca) in normal pregnant uterine smooth muscle cells and uterine smooth muscle cells after interleukin-6 (IL-6) treatment Inhibition of Atractylodes on Uterine Contraction in Preterm Labor and Its Electrophysiological Mechanism. Methods: ① Acute isolation of human uterine smooth muscle cells from late pregnancy, using the Axopatchl-D patch clamp amplifier to measure the large conductance of single smooth muscle cell calcium channel-dependent potassium channel current (BKca) in whole cell model; Of Atractylodes macrocephala, changes of uterine smooth muscle cells BKca; ③ determination of perfusate containing IL-6 (10ng / ml) in whole-cell mode BKca; then measured the perfusion fluid containing different concentrations of Atractylodes macrocephala, changes in uterine smooth muscle cells BKca . Results: The amplitude of BKca was about 36.9 ± 13.7% (n = 10, P <0.01) with 10ng / ml IL-6 and 36.7 ± 22.6% % (n = 10, P <0.01). For the cells treated with IL-6, the amplitude of BKca enhanced by 2mg.mL-1 atractylodes rhizome was about (45.2 ± 13.7)% (n = 10, P <0.01). Conclusion: Atractylodes macrocephalae can excite BKca of uterine smooth muscle cells and IL-6-treated uterine smooth muscle cells in late pregnancy, and it is more effective in stimulating BKca of uterine smooth muscle cells after IL-6 treatment than that of normal uterine smooth muscle cells To maintain the membrane potential and resting state of uterine smooth muscle during pregnancy, to provide some theoretical basis for the development of traditional Chinese medicine treatment of premature delivery.