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AIM:To investigate the expression of cancer-testis (CT)antigens MAGE-1,SSX-1 ,CTpll and HCA587 genes inhepatocellular carcinoma (HCC) and the possibility ofapplying these anUgens as targets for specific immunotherapyfor HCC.METHODS:Expression levels of MAGE-1,SSX-1,CTp11and HC4587mRNA were detected with reverse transcriptionpolymerase chain reaction (RT-PCR) in HCC tissues andcorresponding adjacent non-cancerous tissues from 105HCC patients,40 samples of cirrhosis and normal liver tissues.Genes of five samples with positive PCR results weresequenced.RESULTS:Of 105 HCC tissues,MAGE1,SSX-1 ,CTp11 andHCA587mRNA expressions were detectable in 75.2%(79/105),72.4%(76/105),62.9%(66/105) and 56.2%(59/105) of HCCsamples,respectively.About 93.3%(98/105),72.4%(76/105),48.6%(51/105) and 37.1%(39/105) of HCC tissues positivelyexpressed at least one,two,three,and four members of CTantigens,respectively.Conversely,only SSX-1 could bedetectable in 2.9%(3/105) of the corresponding adjacentnon-HCC tissues in which no metastatic lesion was found.Of the latter 3 patients,biopsy samples far from tumorwere obtained in 2 patients and RT-PCR indicated noexpression of SSX-1 mRNA in these two samples.In addition,none of 40 samples of cirrhotic and normal liver tissuesexpressed CT antigen gene mRNA.DNA sequencesconfirmed that the RT-PCR products were true targetcDNA.No relationship was found between expression of CTantigens and clinico pathological indicators such as age,gender,tumor size,degree of tumor differentiation,serumα-fetoprotein level and infecUon of hepatitis B virus or hepatitisC virus (P>0.05).CONCLUSION:CT antigens genes (MAGE-1,SSX-1,CTp11and HCA587) are expressed with high percentage andspecificity in HCC and their products are promising targetsfor antigen-specific irnmunotherapy of HCC.High frequentco-expression of multiple members of CT antigens in HCC provides possibility of polyvalent vaccinations for HCC.
AIM: To investigate the expression of cancer-testis (CT) antigens MAGE-1, SSX-1, CTpll and HCA587 genes in hepatocellular carcinoma (HCC) and the possibility of appling these an Ugens as targets for specific immunotherapy for HCC. METHODS: Expression levels of MAGE -1, SSX-1, CTp11 and HC4587 mRNA were detected with reverse transcription polymerase chain reaction (RT-PCR) in HCC tissues and corresponding to adjacent non-cancerous tissues from 105 HCC patients, 40 samples of cirrhosis and normal liver tissues. Genes with five samples with positive PCR Results were sequenced. RESULTS: Of 105 HCC tissues, MAGE1, SSX-1, CTp11 and HCa587 mRNA expressions were detectable in 75.2% (79/105), 72.4% (76/105), 62.9% (66/105) and 56.2% / 105) of HCCs samples respectively, 93.3% (98/105), 72.4% (76/105), 48.6% (51/105) and 37.1% (39/105) of HCCs were positively expressed at least one, two, three, and four members of CTantigens, respectively. Conversely, only SSX-1 could be undetectable in 2.9% (3/105) of the corresponding adjacent non-HCC tissu es in which no metastatic lesion was found. Of the latter 3 patients, biopsy samples far from tumorwere obtained in 2 patients and RT-PCR indicated no expression of SSX-1 mRNA in these two samples. In addition, none of 40 samples of cirrhotic and Normal liver tissuesexpressed CT antigen gene mRNA. DNA sequences confirmed that the RT-PCR products were true of the expression of CTantigens and clinico pathological indicators such as age, gender, tumor size, degree of tumor differentiation, serum alpha-fetoprotein level and infecUon of hepatitis B virus or hepatitisC virus (P> 0.05) .CONCLUSION: CT antigens genes (MAGE-1, SSX- 1, CTp11 and HCA587) are expressed with high percentage and specificity in HCC and their products are promising targets for antigen- specific irnmunotherapy of HCC. High frequentco-expression of multiple members of CT antigens in HCC provides possibility of polyvalent vaccinations for HCC.