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目的探究肝脏不同c DCs亚群的特性和抗原递呈能力。方法分离肝脏非实质细胞和脾脏混合细胞,流式分选得到肝脏CD103~+ cDC(脾脏内CD8α~+ cDC)和CD11b~+ cDC,Q-PCR检测不同cDCs亚群特征性分子的表达。分离OT-Ⅰ/OT-Ⅱ转基因小鼠na?ve T细胞,并将得到的T细胞进行CFSE标记。将DCs和CFSE标记的T细胞加入特异的OVA肽段,体外共培养3 d,收集细胞检测T细胞增殖。结果稳态下小鼠肝脏内的CD103~+ cDC和CD11b~+ cDC类似脾脏CD8α~+ cDC和CD11b~+ cDC,分别负责活化CD8~+T细胞和CD4~+T细胞。结论发现了肝脏DCs的分群特性,以及证明了肝脏内cDC不同亚群对不同T细胞的活化作用,为进一步研究肝脏DCs的功能奠定基础。
Objective To investigate the characteristics and antigen presenting ability of different subpopulation of c DCs in liver. Methods CD103 ~ + cDC (CD8α ~ + cDC in spleen) and CD11b ~ + cDC in liver were isolated by flow cytometry. The expression of molecular markers in different subsets of cDCs was detected by Q-PCR. The OT-Ⅰ / OT-Ⅱ transgenic mouse na? Ve T cells were isolated and the resulting T cells were labeled with CFSE. DCs and CFSE-labeled T cells were added to the specific OVA peptide and co-cultured for 3 days in vitro. The cells were harvested for T cell proliferation. Results CD103 ~ + cDCs and CD11b ~ + cDCs in the liver of mice at steady state were similar to CD8α ~ + cDCs and CD11b ~ + cDCs in spleen, which were responsible for activation of CD8 ~ + T cells and CD4 ~ + T cells, respectively. Conclusion The clustering characteristics of liver DCs were found, as well as the activation of different T cells in different subpopulations of cDCs in liver was proved, which laid the foundation for further study on the function of liver DCs.