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乙醇提取刺齿凤尾蕨药材,提取物依次经过有机溶剂萃取,聚酰胺柱色谱、葡聚糖凝胶柱色谱和制备型HPLC色谱分离得到10个对映-贝壳杉烷型二萜类化合物。采用核磁共振(NMR)和质谱(ESI-MS)技术鉴定获得的化合物分别为geopyxin B(1),geopyxin E(2),ent-11α-hydroxy-18-acetoxykaur-16-ene(3),ent-14β-hydroxy-18-acetoxykaur-16-ene(4),neolaxiflorin L(5),ent-3β,19-dihydroxy-kaur-16-ene(6),ent-3β-hydroxy-kaur-16-ene(7),7β,17-dihydroxy-16α-ent-kauran-19-oic acid 19-O-β-Dglucopyranoside ester(8),crotonkinin C(9)和crotonkinin C(10)。化合物1~10均为首次从刺齿凤尾蕨中获得。体外活性研究表明,化合物1和2对Bel-7402细胞具有抑制作用,IC50分别为7.50,10.60μmol·L-1;化合物1和2对Hep G2细胞具有抑制作用,IC50分别为6.68,11.80μmol·L-1。
Ethanol extraction of Prickly heat fern medicinal herbs, extracts were followed by organic solvent extraction, polyamide column chromatography, Sephadex column and preparative HPLC chromatographic separation of 10 enantiomer - Kauri diterpenoid compounds. The compounds obtained by NMR and ESI-MS were geopyxin B (1), geopyxin E (2), ent-11α-hydroxy-18-acetoxykaur-16-ene -14β-hydroxy-18-acetoxykaur-16-ene (4), neolaxiflorin L (5), ent-3β, 19-dihydroxy-kaur-16-ene (7), 7β, 17-dihydroxy-16α-ent-kauran-19-oic acid 19-O-β-Dglucopyranoside ester (8), crotonkinin C (9) and crotonkinin C (10). Compounds 1 to 10 were obtained for the first time from the spiny fern. In vitro activity studies showed that compounds 1 and 2 had inhibitory effects on Bel-7402 cells with IC50 of 7.50,10.60μmol·L-1, respectively. Compounds 1 and 2 had inhibitory effects on Hep G2 cells with IC50 of 6.68 and 11.80μmol · L-1.