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1977年Yolken首先应用酶联免疫吸附试验(ELISA)检测轮状病毒抗原取得成功,为轮状病毒快速诊断开辟了新的途径。我院于1982～1984年的秋季采集沈阳地区婴幼儿腹泻粪便标本,用ELISA方法检测轮状病毒抗原,同时做部分粪便的细菌培养和病毒分离。
In 1977, Yolken first used the enzyme-linked immunosorbent assay (ELISA) to test the success of the rotavirus antigen, opening up a new way for the rapid diagnosis of rotavirus. Our hospital collected the diarrhea stool samples of infants and young children in Shenyang in the autumn of 1982 ~ 1984, detected the rotavirus antigen by ELISA method, at the same time made some stool bacterial culture and virus isolation.