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目的:建立大鼠脑脊液中梓醇的含量测定方法。方法:大鼠按5.0 mg.kg-1尾静脉注射1.0 g.L-1梓醇生理盐水注射液,40 m in后,经大脑蛛网膜下腔抽取脑脊液;另抽取正常大鼠脑脊液作为空白对照,置-20℃冰箱保存备用。样品经处理后用HPLC检测。选用Hypersil C18反相色谱柱,流动相为水-乙腈(99.5∶0.5),流速1.0 mL.m in-1,检测波长210 nm。结果:脑脊液中梓醇在0.5~40 mg.L-1浓度线性关系良好,r=0.999 7;测得低、中、高浓度绝对回收率为(90.2±1.71)%,(89.1±1.17)%,(86.9±0.98)%;方法回收率为(99.8±1.98)%,(101.1±3.04)%,(100.1±2.30)%;日内、日间精密度均小于4%,在-20℃下冷冻保存15 d稳定。结论:本方法操作简便,能较准确的测出脑脊液中的梓醇含量。
Objective: To establish a method for the determination of catalpol in rat cerebrospinal fluid. Methods: Rats were injected with 1.0 g L-1 catalpol saline injection via the caudal vein of 5.0 mg · kg-1 for 40 mins. Cerebrospinal fluid (CSF) was extracted from the subarachnoid space of the brain. Normal cerebrospinal fluid (CSF) was taken as blank control -20 ℃ refrigerator spare. Samples were processed for HPLC testing. Hypersil C18 reversed-phase column was used. The mobile phase was water-acetonitrile (99.5: 0.5) and the flow rate was 1.0 mL.m in-1. The detection wavelength was 210 nm. Results: The linearity of catalpol in cerebrospinal fluid was good at a concentration of 0.5-40 mg · L -1, r = 0.999 7. The absolute recoveries of low, medium and high concentrations were (90.2 ± 1.71)% and (89.1 ± 1.17)%, respectively , (86.9 ± 0.98)%, respectively. The recoveries were (99.8 ± 1.98)%, (101.1 ± 3.04)% and (100.1 ± 2.30)%, respectively. The intra- and inter- Save 15 d stable. Conclusion: The method is simple and can measure the catalpol content in cerebrospinal fluid more accurately.