以细胞感染试验、凝血法、交叉免疫电泳等技术 ,观察荧光与亚甲蓝联用对血浆中水泡性口炎病毒 (VSV)和辛德比斯病毒 (SV)的灭活效果及血浆蛋白的变化。结果 ,向盛装在聚氯乙烯袋内的血浆中加 1μmol/L亚甲蓝并经荧光 (30 0 0 0Lux)照射 30min ,可将血浆中滴度 >7TCID50 (lg)的VSV和SV灭活。消毒后 ,血浆中的Ⅷ :C、Ⅸ :C、纤维蛋白原的回收率 >75% ,白蛋白、球蛋白的回收率 >85% ,蛋白免疫原性无变化 To observe the inactivation of plasma vesicular stomatitis virus (VSV) and Sindbis virus (SV) and the changes of plasma proteins by the combination of fluorescence and methylene blue in cell infection test, coagulation and cross immunoelectrophoresis . As a result, VSV and SV with a titer of> 7 TCID50 (1 g) in plasma could be inactivated by adding 1 μmol / L methylene blue to plasma contained in a polyvinyl chloride bag and irradiating with fluorescence (30,000 Lo) for 30 minutes. After disinfection, plasma Ⅷ: C, Ⅸ: C, fibrinogen recovery> 75%, albumin, globulin recovery> 85%, no change in protein immunogenicity