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用20%PEG6000处理湖北海棠(Malus hupehensis Rehd.)根系,研究了水分胁迫条件下根系线粒体膜电位(Δψm)、线粒体H2O2含量、细胞程序性死亡关键酶类caspase-3/7活性的变化。结果显示,在20%PEG6000处理前6d,根系线粒体Δψm缓慢降低,第6天后急剧下降。线粒体H2O2含量在处理的第1～6天里缓慢升高,第6天后则快速上升。TUNEL原位检测显示,PEG6000处理能够使根系切片上出现清晰的阳性反应斑点,且随着处理时间的延长阳性反应斑点增多;处理第3天和第6天,根系中类caspase-3/7活性较低,而处理第9天和第12天时类caspase-3/7活性成倍升高,这表明20%PEG6000能够诱导湖北海棠根系细胞程序性死亡。
The roots of Malus hupehensis Rehd. Were treated with 20% PEG6000 to study the changes of mitochondrial membrane potential (Δψm), mitochondrial H2O2 content and the key enzyme caspase-3/7 activity under water stress conditions. The results showed that mitochondrial Δψm decreased slowly at 6 days before 20% PEG6000 treatment and dropped sharply after 6 days. The contents of mitochondrial H2O2 slowly increased from the first day to the sixth day of treatment and rapidly increased after the sixth day. TUNEL in situ detection showed that PEG6000 treatment could make clear positive reaction spots on root sections, and positive reaction spots increased with the prolongation of treatment time. On the 3rd and 6th day after treatment, caspase-3/7 activity Lower, while the activity of caspase-3/7 cascade increased on day 9 and day 12, indicating that 20% PEG6000 could induce apoptosis in the roots of Begoniacens.