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单克隆抗体的发明,可以说是世界上近年来在生物免疫技术领域里取得的最显著的成就。它运用细胞杂交技术将经抗原激活而产生抗体的脾细胞和能在体内外无限制增殖的小鼠脊椎瘤细胞融合而获得杂交瘤细胞。一旦这样的细胞株建立并克隆化后,就可在动物体内或实验室培养条件下源源不断地生产出单克隆抗体。单克隆抗体除了生产时产率高、耗时省以外,其最大特点是具有高度的特异性。由于它实际上是由单细胞系列产生的同一种抗体蛋白,故与抗原性物质反应时绝对专一,不受其它物质的干扰,能区别出二种物质间的细微差异。把单克隆抗体与放射性同位素和酶相结合发展起来的放
The invention of monoclonal antibody can be said that in the world in recent years in the field of bio-immunization technology made the most significant achievements. It utilizes hybridization technique to obtain hybridoma cells by fusion of antigen-activated antibody-producing spleen cells and mouse spinal tumor cells that can proliferate in vitro and in vivo without restriction. Once such cell lines have been established and cloned, monoclonal antibodies can be produced continuously in animal or laboratory culture conditions. Monoclonal antibodies in addition to the production of high yield, time-consuming province, its most prominent feature is a high degree of specificity. Because it is actually the same antibody protein produced by a single cell series, it is absolutely specific when it comes to reacting with antigenic substances and is immune to interference from other substances and distinguishes the subtle differences between the two substances. Put the monoclonal antibody and radioactive isotopes and enzymes developed in combination