论文部分内容阅读
本研究采用抗肿瘤坏死因子单克隆抗体预先保护实验动物，用ＥＬＩＳＡ法测定血浆中肿瘤坏死因子（ＴＮＦ）浓度，藉以考察ＴＮＦ在内毒素诱导病理性氧供应依赖性（ＰＯＳＤ）模型中是否起介导作用。结果表明，（１）ＴＮＦ浓度内毒素组显著高于对照组，抗体保护组显著低于无关抗体组（Ｐ＜０．０１）。（２）在Ｖｏ＿２－Ｄｏ＿２关系中，抗体保护组可清楚地分为依赖段和非依赖段两部分，其临界氧运输量和合并氧提取率分别为１０．８０±３２１ｍｌ·ｍｉｎ￣（－１）·ｋｇ￣（－１）和０．６９０，而无关抗体组则只有依赖段，合并氧提取率为０．４０８，显著低于抗体保护组。从而证明，ＴＮＦ在内毒素所致的ＰＯＳＤ现象中起介导作用。
In this study, the anti-tumor necrosis factor monoclonal antibody was used to protect experimental animals beforehand, and the level of tumor necrosis factor (TNF) in plasma was determined by ELISA, so as to investigate whether TNF plays a role in endotoxin-induced pathological oxygen supply-dependent Guide role. The results showed that: (1) TNF concentration in endotoxin group was significantly higher than that in control group, antibody protection group was significantly lower than non-related antibody group (P <0.01). (2) In the Vo_2-Do_2 relationship, the antibody protection group can be clearly divided into two parts: dependent and non-dependent, and the critical oxygen transport and combined oxygen extraction rates were 10.80 ± 321ml · min ~ (-1) ) · Kg ~ (-1) and 0.690, while the unrelated antibody group only relies on the fragment, the combined oxygen extraction rate was 0.408, significantly lower than the antibody protection group. Thus, it is demonstrated that TNF plays a mediating role in endotoxin-induced POSD phenomenon.