[目的]筛选对菜地青菜斑枯病原菌具有拮抗作用的细菌。[方法]从各地的土壤中分离到200多株产芽孢细菌,对其进行初筛、复筛,再对抑菌活性较高的产芽孢细菌进行菌体形态、菌落特征的观察及一系列生理生化试验和16SrDNA序列测定。[结果]复筛结果表明,菌株ZG-10、ZG-19、ZG-59、ZG-72、ZG-31有显著的抑菌活性,对青菜斑枯病生物防治具有很大的研究价值和应用前景;菌株ZG-10经种属鉴定为枯草芽孢杆菌(Bacillus subtilis)。[结论]ZG-10菌株具备生物防治潜力和良好的发展前景。该研究为后续研究奠定了一定的基础,也为拮抗菌株与蛋白多肽在农业上的应用开辟了道路。 [Objective] The research aimed to screen the bacteria which antagonized the pathogen of vegetable spot blight in vegetable field. [Method] More than 200 strains of spore-forming bacteria were isolated from soil in different places. The spore-forming bacteria were screened and re-screened. Then the bacteria with high antibacterial activity were observed for morphology and colony characteristics and a series of physiological Biochemical tests and 16S rDNA sequencing. [Results] The results of the double screen showed that the strains ZG-10, ZG-19, ZG-59, ZG-72 and ZG-31 had significant antibacterial activities and had great research value and application on the biological control of the blight The strain ZG-10 was identified as Bacillus subtilis by species. [Conclusion] The strain ZG-10 possesses the potential of biological control and its good prospect of development. The study laid the foundation for further research and opened the way for antagonism of bacterial strains and application of protein peptides in agriculture.