论文部分内容阅读
目的 探讨恶性疟原虫ＤＮＡ 疫苗在小鼠体内的免疫反应。方法 将恶性疟原虫ＦＣＣ－ １／ＨＮ株有性期阶段的重组质粒ｐｃＤＮＡ３－ Ｐｆｓ２５ 经骨骼肌途径注射ＢＡＬＢ／ｃ小鼠，对注射部位的骨骼肌进行了预处理，即于注射前７ｄ 先在左后肢股四头肌注射０．５％ 盐酸布比卡因５０μｌ，注射深度为２ｍ ｍ 。观察免疫后不同时间点小鼠血清ＩｇＧ抗体滴度、脾淋巴细胞增殖反应、ＣＤ４＋ ／ＣＤ８＋ Ｔ细胞亚群比值和ＮＫ 细胞杀伤活性的变化。结果 １）重组质粒ｐｃＤＮＡ３－ Ｐｆｓ２５ 经肌肉注射途径免疫小鼠后，机体ＩｇＧ抗体滴度增高、特异性Ｔ淋巴细胞增殖反应增强、ＣＤ８＋ Ｔ细胞亚群比值增高以及ＮＫ细胞杀伤活性增强。２）肌肉注射为一有效的ＤＮＡ疫苗免疫途径。结论 采用编码有性期基因的重组质粒ｐｃＤＮＡ３－ Ｐｆｓ２５ 经骨骼肌注射途径免疫小鼠，能诱导机体有效的体液免疫、细胞免疫和ＮＫ细胞杀伤活性。本研究为恶性疟ＤＮＡ疫苗的研究提供了免疫学实验依据
Objective To investigate the immune response of Plasmodium falciparum DNA vaccine in mice. Methods BALB / c mice were injected with recombinant plasmid pcDNA3-Pfs25 of Plasmodium falciparum FCC-1 / HN at the sexual stage through the skeletal muscle. The skeletal muscle at the injection site was pretreated, that is, Left hind limb quadriceps injection of 0.5% bupivacaine hydrochloride 50μl, injection depth of 2m m. The changes of serum IgG antibody titers, splenic lymphocyte proliferation, CD4 + / CD8 + T cell subsets and NK cytotoxicity were observed at different time points after immunization. Results 1) The recombinant plasmid pcDNA3-Pfs25 immunized mice by intramuscular injection, the body IgG antibody titer increased, specific T lymphocyte proliferation increased, CD8 T cell subsets ratio increased and NK cell cytotoxic activity increased. 2) Intramuscular injection is an effective DNA vaccine immunization pathway. Conclusion Immunization of mice with a recombinant plasmid pcDNA3-Pfs25 encoding sexually transmitted genes via skeletal muscle injection can induce effective humoral, cellular and NK cell killing activities. This study provides an experimental basis for the immunization of Plasmodium falciparum DNA vaccine research