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目的对青海省1958-2013年从人体分离到的布鲁氏菌进行分析。方法对1958年以来,临床诊断为布鲁氏菌病(布病)患者分离出的43株布鲁氏菌的来源、菌培养与临床关系等进行整理分析,硫堇和碱性复红的染料抑菌试验、单项血清凝集反应、噬菌体裂解试验对43株布鲁氏菌进行菌株表型分型鉴定。结果取材337份做布鲁氏菌培养,分离出43株,其中慢性患者15例局部病灶检出布鲁氏菌。43株均为羊种布鲁氏菌,其中3株为羊1型,14株为羊2型,26株为羊3型。结论羊种菌是青海省人群布病的致病菌。
Objective To analyze the Brucella isolated from human in Qinghai from 1958 to 2013. Methods The origin, bacterial culture and clinical relationship of 43 strains of Brucella isolated from patients with brucellosis (brucellosis) clinically diagnosed since 1958 were analyzed. The contents of thionine and basic complex red dye Bacteriostatic test, single serum agglutination test and phage lysis test were performed on 43 strains of Brucella. RESULTS: A total of 337 samples were collected and cultured in Brucella. 43 isolates were isolated, of which Brucella was detected in 15 of the chronic cases. The 43 strains were all Brucella brucei, of which 3 were sheep type 1, 14 were sheep type 2 and 26 were sheep type 3. Conclusion The sheep is the pathogen of brucellosis in Qinghai Province.