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能源树种小桐子属冷敏植物,受低温胁迫影响涉及到形态、生理、生化、基因表达及调控等多个方面。我们最近通过RNA-Seq技术获得了小桐子低温锻炼转录组(45 251条Unigene)及其数字基因表达谱数据,发现在12℃低温锻炼下有4 185个基因表达量发生变化,其中在不同锻炼时间(12 h,24 h,48 h)内都发生变化的基因有553个。本工作从中选择了22个与对照相比有较大表达差异、新诱导的或被公认的抗冷相关基因,通过半定量RT-PCR及进一步聚类分析检测它们在小桐子不同组织及低温锻炼过程中的表达模式,结合其数字基因表达结果以期找出与小桐子抗冷性密切相关的潜在关键基因,为小桐子抗冷性的遗传改良打下基础。
Energy species of Jatropha genus cold-sensitive plants, affected by low temperature stress involves morphology, physiology, biochemistry, gene expression and regulation and many other aspects. We recently obtained the cryogenic exercise Jatropha (45,211 Unigene) transcriptomes and their digital gene expression profiling data by RNA-Seq technology. We found 4,185 gene expression changes under low temperature exercise at 12 ℃. Among them, There were 553 genes that changed in time (12 h, 24 h, 48 h). In this work, we selected 22 genes that had higher expression difference than the control, newly induced or recognized cold-related genes, and detected their expression in different tissues of Jatropha curcas and hypothermia by semi-quantitative RT-PCR and further cluster analysis In the process of expression, combined with the results of digital gene expression in order to identify potential genes closely related to cold resistance in Jatropha, lay the foundation for genetic improvement of cold resistance in Jatropha curcas.