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目的:建立民间用药鸡眼草的质量标准评价方法。方法:采用性状鉴别和叶、茎横切面及粉末的显微鉴别方法鉴别鸡眼草,采用高效液相色谱法测定其中的山柰酚,色谱柱:C18(4.6 mm×150 mm,5μm);流动相:甲醇-0.1%磷酸(63∶37);检测波长:370 nm。结果:在叶、茎横切面显微图可见乳汁管和韧皮纤维束。在粉末显微图中,可见非腺毛、晶纤维束等。采用HPLC法测定鸡眼草中山柰酚的含量,稳定性良好,精密度良好,重复性良好,加样回收率良好,每1 g鸡眼草中山柰酚含量为0.046～0.049 mg。结论:鸡眼草的药材性状、显微鉴别特征明显,对该生药鉴别具有重要意义。HPLC法可用于测定鸡眼草中山柰酚的含量。
Objective: To establish a quality standard evaluation method of folk medicine. METHODS: The traits of tracheid were identified by traits identification and microscopic identification of the cross section of leaves and stems and powder, and the content of kaempferol was determined by high performance liquid chromatography (C18: 4.6 mm × 150 mm, 5 μm) Phase: methanol -0.1% phosphoric acid (63:37); detection wavelength: 370 nm. Results: The latex tube and bast fiber bundles were observed in the micrographs of the leaf and stem cross-sections. In the powder micrograph, visible non-glandular hair, crystal fiber bundle. The content of kaempferol was determined by HPLC. The stability was good, the precision was good, the repeatability was good and the recovery rate was good. The content of kaempferol per 1 g of Cornice was 0.046 ~ 0.049 mg. Conclusion: The characteristics of medicinal materials and microscopic identification of Cornflower are obvious, which is of great significance for the identification of this crude drug. HPLC method can be used to determine kudzu grass kaempferol content.