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采用琼脂平板扩散法筛选对番茄灰霉菌具有拮抗作用的细菌,经过对200株分离自土壤的细菌菌株进行初筛、复筛后,得到了1株具有较高拮抗活性的菌株X-75。对该菌株进行形态观察和生理生化特征分析,初步将其鉴定为芽孢杆菌属(Bacillus sp.),将其16SrDNA序列与GenBank中已知标准菌株的16SrDNA序列进行比对,并用Neighbor-joining方法构建X-75菌株进化树,结果表明,X-75与标准菌株AB245422 Bacillus velezensis聚于同一分支,同源性最高,达99.93%;利用番茄离体叶片对X-75菌株的防效进行初步检测,结果表明X-75菌株对番茄灰霉病菌具有明显的拮抗作用。
An agar plate diffusion method was used to screen the bacteria that had antagonistic effect on Botrytis cinerea. After screening and isolating 200 bacterial strains isolated from soil, one strain X-75 with high antagonistic activity was obtained. The morphological and physiological and biochemical characteristics of the strain were identified. The strain was initially identified as Bacillus sp., And its 16S rDNA sequence was aligned with the 16S rDNA sequence of the known standard strain in GenBank and constructed using the Neighbor-joining method X-75. The results showed that X-75 and Bacillus velezensis were clustered in the same branch with the highest homology of 99.93%. The control effect of X-75 strain on tomato leaves was preliminarily detected, The results showed that X-75 strain had obvious antagonism against Botrytis cinerea.