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Two new eremophilenolides were isolated from the roots of Ligularia muliensis. Their structures were elucidated on the basis of the spectroscopic analysis as 6β-acetoxy-8β-hydroxyl-eremophil-7(11)-en-12,8α-olide 1 and 6α-hydroxy-8βH-eremophil-7(11)-en-12,8α-olide 2.