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在现有的心肌梗塞血清学诊断试验中,血清肌酸激酶-MB同功酶测定是最灵敏和特异的方法;然而,由BB同功酶制备的抗血清对大脑的肌酸激酶也起反应,如同时有卒中或是卒中而不是心肌梗塞,可引起混淆。肌球蛋白测定由于骨骼肌和心肌的肌球蛋白在结构和免疫学上相一致,当骨胳肌受到损伤时,亦可产生混淆。心肌肌凝蛋白轻链(CM-LC)的结构和免疫学特性则与骨胳肌肌凝蛋白轻链不同,是心脏的特异性抗原。本文报道一种血清CM-LC的放射免疫测定方法,以及实验狗急性心肌梗塞后的血清CM-LC测定结果。方法:CM-LC的制备是先按Katz等报道的方法从狗左心室肌提取心肌肌凝蛋白。纯化的肌凝蛋白沉淀在10容积的冷蒸馏水中。然后按Perrie
Serum creatine kinase-MB isozyme assay is the most sensitive and specific method in existing myocardial infarction serological diagnostic tests; however, antisera prepared by BB isozyme also react with creatine kinase in the brain If there is a stroke or stroke at the same time instead of a myocardial infarction, it can cause confusion. Myosin Assays Because skeletal and cardiac myosin are structurally and immunologically consistent, confusion can also occur when skeletal muscle is damaged. The structural and immunological properties of cardiac myosin light chain (CM-LC) are different from that of skeletal muscle myosin light chain and are heart-specific antigens. In this paper, a method for the determination of serum CM-LC by radioimmunoassay and the result of serum CM-LC after acute myocardial infarction in experimental dogs were reported. Methods: CM-LC was prepared by first extracting the cardiac myosin from the left ventricular muscle of a dog as reported by Katz et al. Purified myosin was precipitated in 10 volumes of cold distilled water. Then press Perrie