胆汁酸是一类结构相近的甾体化合物,常用薄层层析法分离。显示它们在层析谱上的位点一般仍采用碘蒸气或磷钼酸显色,但难于根据斑点色泽区别各种胆汁酸种类;脂溶性有机化合物电容易造成伪斑点。芳香醛类与胆汁酸能形成特征性颜色。我们系统地观察了一些芳香醛与常见的10种胆汁酸的颜色反应及其荧光现象,其中4-硝基苯甲醛,3,4-甲烯二氧苯甲醛用于对胆汁酸的检测尚未见报道。 Bile acid is a kind of steroidal compound with similar structure, and is usually separated by thin layer chromatography. Their positions on the chromatogram are generally still expressed with iodine vapor or phosphomolybdic acid, but it is difficult to distinguish bile acid species based on the spot color; fat-soluble organic compounds can easily cause pseudo spots. Aromatic aldehydes and bile acids can form characteristic colors. We systematically observed the color reaction and fluorescence phenomenon of some aromatic aldehydes and common 10 bile acids. Among them, 4-nitrobenzaldehyde and 3,4-dimethylenedioxybenzaldehyde have not been used to detect bile acids. Reported.