侵染豆科植物的种传病毒大多由种胚或了叶带毒,翌年随种子萌发进入幼苗体内,造成感染,在有条件的地方可用ELISA,RIA法等直接检测种胚或子叶带毒情况。但在缺乏血清,不具备必要设备的地方,不妨一试本方法。 用灭菌水浸泡5-10粒种子,24小时后切取小块子叶,加磷酸缓冲液研磨捣碎,用常规摩擦接种指示植物。剩下大部分带胚种子 Most of the virus inoculated with leguminous plants is germinated or leaf poisoned. The following year germinated with the seed into the seedling, causing infection. Where necessary, ELISA and RIA methods can be used to directly detect the embryo or cotyledon poisoning . But in the absence of serum, do not have the necessary equipment, may wish to try this method. Soak 5-10 seeds with sterilized water, cut the cotyledons 24 hours later, add the phosphate buffered saline to the ground, and instruct the plants with conventional rubbing inoculation. Most of the remaining germ seeds