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将转入球形芽孢杆菌毒蛋白基因表达质粒的工程藻Ａｎａｂａｅｎａｓｕｂｔｒｏｐｉｃ（ｐＤＣ２６）大量培养后，应用于山东省平阴县进行现场观察。结果表明：工程藻在９．４１×１０５ｃｅｌｌｓ／ｍｌ浓度以上时，４８ｈ可杀死９９～１００％蚊幼虫；在９．１３×１０４ｃｅｌｌｓ／ｍｌ浓度时，４ｄ可杀死９９％以上蚊幼虫，连续观察２个多月，工程藻仍有明显杀蚊效果，实验组最多１４条／勺幼虫，而对照组２１０条／勺左右。观察还发现，工程藻对库蚊幼虫杀灭效果最好，其次为伊蚊，但高浓度时，对伊蚊仍有明显杀灭效果；由于按蚊幼虫密度太低，未能观察。此结果比单独使用球形芽孢杆菌杀灭蚊幼虫有明显提高，持续时间也延长，这将为蚊虫的持续控制提供一个新的有力武器。
The engineering bacterium Anabaenasubtropic (pDC26) which was transformed into the Bacillus sphaerubicin gene expression plasmid was extensively cultured and applied to the field observation in Pingyin County, Shandong Province. The results showed that 99 ~ 100% of mosquito larvae could be killed 48h after the engineering algae was above 9.41 × 10 5 cells / ml, and more than 99% of mosquito larvae could be killed after 4 days at the concentration of 9.13 × 104 cells / ml Observation of more than two months, the engineering algae still significant mosquito effect, the experimental group up to 14 / spoon larvae, while the control group of 210 / spoon. The observation also found that the engineering algae had the best killing effect on Culex pipiens larvae, followed by Aedes albopictus, but at high concentrations, there was still a significant killing effect on Aedes albopictus. Because the density of Anopheles larvae was too low, it could not be observed. This result is significantly higher than that of using B. sphaericus alone to kill mosquito larvae, and the duration is prolonged. This will provide a new and powerful weapon for the continuous control of mosquitoes.