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流行病学、血清学和分子生物学的资料证实，爱泼斯坦－巴尔病毒（ＥＢＶ感染与鼻咽癌（ＮＰＣ）有密切关系。血清ＥＢＶ－ＶＣＡ－ＩｇＡ，ＥＢＶ－ＥＡ－ＩｇＡ测定和聚合酶链（ＰＣＲ）检测组织中ＥＢＶ－ＤＮＡ已用于ＮＰＣ的临床诊断。本文评价上述方法在ＮＰＣ诊断中的实用价值。８５例病人双盲法测定血清ＥＢＶ－ＶＣＡ－ＩｇＡ（Ｉ），ＥＢＶ－ＥＡ－ＩｇＡ（Ⅱ）：鼻咽活检组织用ＰＣＲ法检测ＥＢＶ－ＤＮＡ（Ⅲ）和组织病理检测（Ⅳ）。组织病理学证实：ＮＰＣ５７例；非ＮＰＣ２８例作为对照组。５７例ＮＰＣ中，（Ⅰ）、（Ⅱ）、（Ⅲ）的阳性率分别为６６．７％，２１．１％，８０．７％；（Ⅰ）＋（Ⅱ），（Ⅰ）＋（Ⅲ），（Ⅱ）＋（Ⅲ）或（Ⅰ）＋（Ⅱ）＋（Ⅲ）的阳性率分别为２１．１％，５６．１％，１９．３％，１９．３％（Ｐ＜０．００５）。２８例对照组（Ⅰ）、（Ⅱ）或（Ⅲ）的阴性率分别为７５％，１００％，１００％；（Ⅰ）＋（Ⅱ），（Ⅰ）＋（Ⅲ），或（Ⅰ）＋（Ⅱ）＋（Ⅲ）均为对．４％（Ｐ＜０．００５）。本组结果提示，ＰＣＲ法检测ＥＢＶ－ＤＮＡ具有灵敏度高（ＮＰＣ的阳性率高）和特异性强（对照组的阴性率高）的特点。
Epidemiological, serological and molecular biological data confirm Epstein-Barr virus (EBV infection is closely related to NPC.) Serum EBV-VCA-IgA, EBV-EA-IgA assay and polymerase (EBV-DNA) has been used in the clinical diagnosis of NPC.This article evaluates the practical value of the above method in the diagnosis of NPC.Methods: Eighty-five patients were tested for the serum levels of EBV-VCA-IgA (I), EBV-EA IgA (Ⅱ): EBV-DNA was detected by PCR in nasopharyngeal biopsies (Ⅲ) and histopathological examination (Ⅳ) .Histopathology confirmed that 57 NPC were NPC patients, ), (Ⅱ) and (Ⅲ) were 66.7%, 21.1% and 80.7% respectively; (Ⅰ) + Ⅲ) or (Ⅰ) + (Ⅱ) + (Ⅲ) were 21.1%, 56.1%, 1 .3% and 19.3%, respectively (P <0.005) .The negative rates of (Ⅰ), (Ⅱ) and (Ⅲ) in the 28 cases were 75%, 100% and 100% (P <0.005) .This group of results suggest that PCR detection of EBV-DNA has the sensitivity High (high positive rate of NPC) and strong specificity (negative rate of the control group).