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目的研究血管内皮生长因子受体-2(VEGFR-2)在皮肤基底细胞癌(BCC)组织中的表达情况。方法提取BCC组织的mRNA,以RT-PCR法检测标本中VEGFR-2 mRNA的表达。同时应用蛋白质印迹法检测VEGFR-2蛋白的表达,免疫荧光法检测VEGFR-2在BCC组织中的定位。结果BCC组织标本mRNA和蛋白水平中均可以检测到VEGFR-2的表达,并较正常人对照有显著增加。VEGFR-2在BCC细胞主要表现为膜性分布。结论皮肤BCC组织细胞能够高异常表达VEGFR-2,可能是导致BCC细胞肿瘤性增殖的原因之一。
Objective To study the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) in human basal cell carcinoma of the skin (BCC). Methods The mRNA of BCC tissues was extracted and the expression of VEGFR-2 mRNA was detected by RT-PCR. At the same time, the expression of VEGFR-2 protein was detected by Western blotting, and the localization of VEGFR-2 in BCC was detected by immunofluorescence. Results The expression of VEGFR-2 was detected both in mRNA and protein level of BCC tissue samples, which was significantly higher than that of normal controls. VEGFR-2 mainly showed membranous distribution in BCC cells. Conclusion The high expression of VEGFR-2 in skin BCC cells may be one of the reasons leading to the tumorigenicity of BCC cells.