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目的 应用免疫阳转血清对HIV抗体检测试剂质量进行评价。方法 采用HIV-1核心区p24、gp41、膜区gp120和全病毒抗原免疫家兔制备系列兔血清,评价国内外HIV抗体双抗原夹心法试剂的质量。结果免疫阳性血清可用于测定试剂对各不同区域抗体检测的能力。结论 对评价试剂盒敏感性有实际意义。
Objective To evaluate the quality of HIV antibody test by immunoprecipitation. Methods A series of rabbit serum was prepared by immunization of rabbits with HIV-1 core region p24, gp41, membrane gp120 and whole virus antigens to evaluate the quality of sandwich ELISA reagents. Results Immuno-positive sera can be used to determine the ability of an agent to detect antibodies in various regions. Conclusion The evaluation of the sensitivity of the kit has practical significance.