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目的探讨癫痫发生过程中突触素Ⅰ(SYNⅠ)在海马和齿状回的表达及齿状回苔藓纤维出芽的动态变化。方法建立匹罗卡品癫痫持续状态模型,用图像分析系统测定海马和齿状回不同时点SYNⅠ免疫反应吸光度值．Neo-timms’染色观察齿状回苔藓出芽的演变。结果SYNⅠ在海马和齿状回的表达于癫痫状态后2d、7d出现降低,14d开始升高,30d、60d表达明显增高；齿状回内分子层于14d开始出现苔藓纤维出芽,大鼠在同期开始出现自发发作。结论在癫痫状态后2d即出现了突触可塑性的变化,14d后由于神经轴突的再生,齿状回内分子层出现苔藓纤维出芽,形成了兴奋性的环路,可能是癫痫反复自发发作的病理基础,SYNⅠ及苔藓纤维出芽较好的反应了神经可塑性的变化。
Objective To investigate the expression of synaptophysin Ⅰ (SYN Ⅰ) in hippocampus and dentate gyrus and the dynamic changes of sprouting mossy fiber during epilepsy. Methods The status epilepticus model of pilocarpine was established, and the SYN I immunoreactivity of hippocampus and dentate gyrus at different time points was measured by image analysis system. Neo-timms’ staining to observe the evolution of germination of dentate moss. Results The expression of SYNⅠ in hippocampus and dentate gyrus decreased at 2d and 7d after epilepsy, increased at 14d, and increased at 30d and 60d. The mossy fiber sprouting appeared in the inner layer of dentate gyrus at 14d, Spontaneous onset began to appear. Conclusions Synaptic plasticity changes occur 2d after epileptic state. After 14 days, due to axonal regeneration, mossy fiber sprouting occurs in the molecular layer of dentate gyrus, forming an excitatory loop, which may be caused by recurrent spontaneous epilepsy Pathological basis, SYNI and moss fiber budding better reflect the changes in neuroplasticity.