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目的:研究从手掌参中分离的Militarine对离体大鼠胸主动脉环的舒张作用并探讨其可能的机制。方法:记录去甲肾上腺素(NE)和KCl预收缩的离体大鼠主动脉环张力变化,观察Militarine的舒血管作用及不同工具药对其作用的影响。结果:Militarine可以抑制去甲肾上腺素(NE,1×10-4mol·L-1)和KCl(1.8 mol·L-1)引起去内皮和内皮完整的离体大鼠胸主动脉环的收缩作用,但二者有明显差别;L-硝基精氨酸甲酯(LNAME,1×10-4mol·L-1)、亚甲蓝(MB,1×10-5mol·L-1)、吲哚美辛(INDO,1×10-5mol·L-1)均能一定程度抑制Militarine对胸主动脉环的舒张作用;钾离子通道阻断剂四乙基胺(TEA,1×10-3mol·L-1)、格列苯脲(glibenclamide,1×10-5mol·L-1)、Ba Cl2(1×10-4mol·L-1)可明显减弱Militarine对血管环的舒张作用;在无钙环境下,预孵育Militarine对去甲肾上腺素引起的血管环收缩有明显抑制作用。结论:Militarine有内皮依赖性和非内皮依赖性的血管舒张作用,其作用机制可能涉及激活内皮细胞中NO/c GMP通路,开放钾离子通道和抑制钙离子内流。
OBJECTIVE: To study the vasodilatory effect of Militarine isolated from the spleen of Panax notoginseng on the thoracic aorta ring of isolated rat and its possible mechanism. Methods: Tension changes of the aortic rings of isolated rat with norepinephrine (NE) and KCl precontracted were recorded. The vasodilatory effects of Militarine and the effects of different tools and drugs on its effects were observed. RESULTS: Militarine inhibited the contractile effects of norepinephrine (NE, 1 × 10-4 mol·L-1) and KCl (1.8 mol·L-1) on intact endothelium and endothelium in isolated thoracic aortic rings (LNAME, 1 × 10-4 mol·L-1), methylene blue (MB, 1 × 10-5 mol·L-1), indole Indomethacin (IND) (1 × 10-5mol·L-1) could inhibit the relaxation of Militarine to the thoracic aortic rings to a certain extent. The potassium channel blocker tetraethylamine (TEA, 1 × 10-3mol·L -1), glibenclamide (1 × 10-5mol·L-1) and BaCl2 (1 × 10-4mol·L-1) could significantly attenuate the vasodilating effect of Militarine on the vascular rings. In the calcium-free environment , Preincubation of Militarine significantly inhibits noradrenaline-induced vasoconstriction. CONCLUSION: Militarine has endothelium-dependent and endothelium-independent vasodilatation. Its mechanism may involve activation of NO / c GMP pathway in endothelial cells, opening of potassium channels and inhibition of influx of calcium ions.