论文部分内容阅读
为探讨魔芋葡甘寡聚糖(K-GLMOS)和瓜胶豆半乳甘露寡聚糖(CB-GAMOS)作为鱼用抗生素生长促进剂替代品的可能性,将270尾同塘同规格的尼罗罗非鱼鱼种随机等分为3组。对照组喂商品罗非鱼种料;K-GLMOS组和CB-GAMOS组分别在饲料中添加1 000mg/kg的K-GLMOS和CB-GAMOS。饲喂28d后,观测鱼的增重率和料肉比;第30～45天测定不同处理组攻击凡隆气单胞菌BJCP-9株后的相对成活率,扫描电镜观察BJCP-9对肠道上皮的黏附;cell-ELISA法体外测试K-GLMOS和CB-GAMOS对BJCP-9黏附罗非鱼肠上皮的影响。结果显示,饲料中添加1 000mg/kg的K-GLMOS和CB-GAMOS显著促进了罗非鱼种的生长,降低了料肉比,并增强了抗BJCP-9感染的能力(P<0.05);1g/L的K-GLMOS或CB-GAMOS虽然均不能抑制BJCP-9的生长,但在体内、外均能抑制BJCP-9对罗非鱼肠道上皮细胞的黏附。5、50、500μg/mL的K-GLMOS和CB-GAMOS在离体模拟黏附试验中,表现出剂量依赖性的抗黏附作用(P<0.05)。表明K-GLMOS和CB-GAMOS作为鱼用抗生素类生长促进剂替代品具有广阔的前景,且抗黏附是其促生长和抗感染的机理之一。
In order to explore the possibility of K-GLMOS and CB-GAMOS as alternatives to fish growth promoters, 270 samples of the same size Tilapia fish were randomly divided into three groups. The control group was fed with tilapia species; K-GLMOS group and CB-GAMOS group were fed with 1000mg / kg K-GLMOS and CB-GAMOS respectively. After feeding for 28 days, the weight gain rate and feed-meat ratio of the fish were observed. The relative survival rates of different treatment groups were tested after 30 days to 45 days. The survival rate of BJCP-9 strain was observed by scanning electron microscopy. The adhesion of the epithelial cells was measured by cell-ELISA. The effects of K-GLMOS and CB-GAMOS on the adhesion of BJCP-9 to the intestinal epithelium of tilapia were tested in vitro. The results showed that the addition of 1 000 mg / kg K-GLMOS and CB-GAMOS significantly promoted the growth of tilapia species, reduced the feed-to-meat ratio and enhanced the resistance to BJCP-9 infection (P <0.05). Although 1g / L K-GLMOS or CB-GAMOS could not inhibit the growth of BJCP-9, BJCP-9 could inhibit the adhesion of BJCP-9 to intestinal epithelial cells of tilapia in vitro and in vivo. K-GLMOS and CB-GAMOS at 5, 50, 500μg / mL showed a dose-dependent anti-adhesion effect (P <0.05) in vitro simulated adhesion test. It shows that K-GLMOS and CB-GAMOS have broad prospects as fish substitutes for growth promotants of antibiotics, and anti-adhesion is one of the mechanisms of their growth-promoting and anti-infective.